Journal: Molecular Cancer

Article Title: DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines

doi: 10.1186/1476-4598-7-15

Figure Lengend Snippet: Expression analysis of methylation-sensitive genes in human breast cancer cell lines . (A) Representative agarose gels of RT-PCR products from CST6, LCN2, SCNN1A, CDH1, CEACAM6, ESR1 , and β-actin . The source of cDNA template is identified for each lane. Normal breast epithelial MCF12A cells represent the positive control cell line. (B) Quantitative real-time PCR results for CST6, LCN2, SCNN1A, CDH1, CEACAM6 , and ESR1 . Black bars correspond to hypermethylator cell lines, cross-hatched bars correspond to low-frequency methylator cell lines, and the white bar (far right) corresponds to MCF12A cells (index control cell line). The expression level of each gene is depicted relative to that of MCF12A cells. Error bars represent S.E.M. Instances of no detectable level of quantitative real-time PCR expression are indicated by an asterisk (*). (C) Unsupervised cluster analysis for 48 genes that are expressed at a detectable level in MCF12A cells. The 12 breast cancer cell lines group into two distinct clusters, designated cluster I (corresponding to hypermethylator cell lines) and cluster II (corresponding to low-frequency methylator cell lines).

Article Snippet: Human breast cancer cell lines BT20 (ATCC# HTB19), BT549 (HTB122), Hs578T (HTB126), MCF7 (HTB22), MDA-MB-231 (HTB26), MDA-MB-415 (HTB128), MDA-MB-435S (HTB129), MDA-MB-436 (HTB130), MDA-MB-453 (HTB131), MDA-MB-468 (HTB132), SKBR3 (HTB30), and ZR-75-1 (CRL-1500) were obtained from the Tissue Culture Core Facility of the University of North Carolina Lineberger Comprehensive Cancer Center (Chapel Hill, NC), and the normal breast epithelial cell line MCF12A [ ] (CRL-10782) was obtained from the American Type Culture Collection [ ].

Techniques: Expressing, Methylation, Reverse Transcription Polymerase Chain Reaction, Positive Control, Real-time Polymerase Chain Reaction, Control

Journal: Molecular Cancer

Article Title: DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines

doi: 10.1186/1476-4598-7-15

Figure Lengend Snippet: Analysis of DNA methyltransferase enzymes among putative hypermethylator and low-frequency methylator cell lines . Results from triplicate determination of total DNMT activity and individual DNMT protein assays are shown. Hypermethylator cell lines are represented by black bars, low-frequency methylators are represented by cross-hatched bars, and MCF12A cells are represented by a white bar. Error bars represent S.E.M. One unit of DNMT activity or DNMT protein level corresponds to the equivalent amount of activity or protein expressed in MCF12A cells. (A) Total DNMT enzymatic activity; (B) DNMT1 protein; (C), DNMT3a protein; and (D), DNMT3b protein.

Article Snippet: Human breast cancer cell lines BT20 (ATCC# HTB19), BT549 (HTB122), Hs578T (HTB126), MCF7 (HTB22), MDA-MB-231 (HTB26), MDA-MB-415 (HTB128), MDA-MB-435S (HTB129), MDA-MB-436 (HTB130), MDA-MB-453 (HTB131), MDA-MB-468 (HTB132), SKBR3 (HTB30), and ZR-75-1 (CRL-1500) were obtained from the Tissue Culture Core Facility of the University of North Carolina Lineberger Comprehensive Cancer Center (Chapel Hill, NC), and the normal breast epithelial cell line MCF12A [ ] (CRL-10782) was obtained from the American Type Culture Collection [ ].

Techniques: Activity Assay

Journal: Molecular Cancer

Article Title: DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines

doi: 10.1186/1476-4598-7-15

Figure Lengend Snippet: DNMT activity levels in breast cancer cell lines correlate with DNMT3b expression . Association between DNMT total activity and DNMT3b protein levels for the 12 breast cancer cell lines and MCF12A cells. Hypermethylator cell lines (black triangles), low-frequency methylator cell lines (white triangles), and MCF12A cells (black circle) demonstrate a statistically significant relationship between DNMT total activity and DNMT3b protein levels.

Article Snippet: Human breast cancer cell lines BT20 (ATCC# HTB19), BT549 (HTB122), Hs578T (HTB126), MCF7 (HTB22), MDA-MB-231 (HTB26), MDA-MB-415 (HTB128), MDA-MB-435S (HTB129), MDA-MB-436 (HTB130), MDA-MB-453 (HTB131), MDA-MB-468 (HTB132), SKBR3 (HTB30), and ZR-75-1 (CRL-1500) were obtained from the Tissue Culture Core Facility of the University of North Carolina Lineberger Comprehensive Cancer Center (Chapel Hill, NC), and the normal breast epithelial cell line MCF12A [ ] (CRL-10782) was obtained from the American Type Culture Collection [ ].

Techniques: Activity Assay, Expressing

Journal: BMC Microbiology

Article Title: Rapid differentiation of Francisella species and subspecies by fluorescent in situ hybridization targeting the 23S rRNA

doi: 10.1186/1471-2180-10-72

Figure Lengend Snippet: Non- Francisella organisms from the strain collection of the Bundeswehr Institute of Microbiology tested to assess the specificity of the FISH assay

Article Snippet: Streptococcus pyogenes , DSMZ 20565 , + , - , -.

Techniques: